Empower Chromatography Core eLearning Track

This track contains 36 modules and covers operation of Empower controlled LC and LC/QDa systems. This track covers the theory and operation of HPLC, UPLC, TUV/PDA detectors and QDa systems. Topics include sample analysis, data analysis and LC method development basics.

If you would like to register for individual eLearning courses, please see courses below:

750000658 Binary vs. Quaternary Gradient Systems
This module discusses two types of solvent delivery systems; binary versus quaternary gradient systems.  It reviews the similarities and differences between the two systems and how to optimize for UPLC gradient chromatography.  The topics covered include hardware components, gradient formation, specification differences, system volume and re-equilibration considerations.

750000788 Achieving the Best LC Peak Shape
Good peak shape is crucial in liquid chromatography for the best sensitivity, peak resolution, and reproducibility of peak areas and retention times. Causes of non-ideal peak shapes and how to improve peak shape are discussed.

750000988 HPLC and UPLC Best Practices and Troubleshooting
This module will cover best practices for HPLC and UPLC users to optimize performance and maximize up-time of the system. The module focuses on prevention and troubleshooting of common LC problems.

750000663 LC Method Development
This module is designed to provide a fundamental understanding of LC method development techniques.

750000773 Practical Tips for UV and PDA Detection
This module will discuss practical tips for optimizing Tunable UV (TUV) and Photodiode Array (PDA) detectors for maximum linearity and sensitivity, rugged and reproducible methods.

750000813 Empower 3 Pro Fundamentals - Introduction and Log in
This module provides an introduction to Empower Software and shows the user how to log into the Pro Interface.

750000814 Empower 3 Pro Fundamentals - Creating an Instrument Method
This module shows the user how to create an instrument method which is needed for data acquistion.

750000816 Empower 3 Pro Fundamentals - Creating a Method Set
This module shows the user how to create a method set which is needed for data acquisition.

750000817 Empower 3 Pro Fundamentals - Creating a Sample Set Method
This module shows the user how to create a sample set method. The sample set method defines what samples will be run and how the data will be collected.

750000818 Empower 3 Pro Fundamentals - Acquiring Data
This module shows the user how to acquire data with Empower Software. This includes making a single injection, loading and running a sample set method, altering a running sample set and queueing multiple sample sets.

750000819 Empower 3 Pro Fundamentals - Theory of Traditional Peak Detection and Integration
This module describes the theory behind traditional peak detection and integration.

750000820 Empower 3 Pro Fundamentals - Theory of Apex Track Peak Detection and Integration
This module describes the theory behind Apex Track peak detection and integration.

750000821 Empower 3 Pro Fundamentals - Creating a Processing Method Using Traditional Algorithm
This module shows how to create a processing method using the traditional algorithm. A processing method is needed to generate results.

750000822 Empower 3 Pro Fundamentals - Creating a Processing Method Using Apex Track Algorithm
This module shows how to create a processing method using the Apex Track algorithm. A processing method is needed to generate results.

750000823 Empower 3 Pro Fundamentals - Reviewing Data within a Processing Method
This module shows how to apply an existing processing method to a sample set without generating any results.

750000824 Empower 3 Pro Fundamentals - Modifying an Existing Processing Method
This module shows how to modify and fine tune an existing processing method.

750000825 Empower 3 Pro Fundamentals - Alter Sample and Processing a Sample Set
This module shows how to use Alter Sample to modify sample information post run and how to process a sample set.

750000826 Empower 3 Pro Fundamentals - Reviewing Results
This module shows how to review a result set including the individual results and calibration curves.

750000827 Empower 3 Pro Fundamentals - Using Report Methods
This module shows how to use an existing report method to preview and print results.

750000828 Empower 3 Pro Fundamentals - Modifying an Individual Report Method
This module shows how to modify an individual report method. Topics include modifying a chromatogram group and a peaks table group.

750000829 Empower 3 Pro Fundamentals - Modifying a Summary Report Method
This module shows how to modify a summary report method. Topics include understanding sorting & grouping and creating a composite group.

750000863 Acquity QDa Processing MS Data with Empower
Overview of Empower 3 FR2 processing of MS data generated from the Acquity QDa as well as other tandem quadrupole mass spectrometers using Empower.

750000864 Acquity Isocratic Solvent Manager
This module covers the operation of the ACQUITY isocratic solvent manager for optimal performance with various Acquity detectors. Emphasis if given to the appropriate splitting and dilution setup for the QDa detector.

750000862 Acquity QDa General Theory and Basic Use
Basic mass spectrometry theory and use of the QDa in Empower 3 Software.

750000678 Theory of HPLC/TUV
This module covers the theory of UV/Visible light absorption and the optics of Waters 2487 and 2489 Tunable Ultra Violet (TUV) detectors for HPLC.  The theory section covers Beer's law, how lamp output, solvent background adsorption, and a compound's spectrum affect chromatography sensitivity and linearity.  The optics section explains the major optical components of the HPLC TUV detectors and how they work.  The module is self paced and contains knowledge self assessment sections.

750000679 Operation of HPLC/TUV
This module covers the operational parameters of Waters 2487 and 2489 Tunable Ultra Violet (TUV) detectors for HPLC.  The module shows how to set the appropriate data acquisition rate and filtering constant for a separation.  There is an explanation and example of using the Median Baseline Filter (MBF) to correct for baseline drifting.  The module is self paced and contains a knowledge self assessment section.

750000680 Maintenance of HPLC/TUV  
This module covers routine daily and yearly maintenance of Waters 2487 and 2489 Tunable UV/Visible (TUV) detectors for HPLC.  This module shows how to properly change the lamp and provides recommended cleaning procedures for the flow cell.  This module is self paced.

750000681 Theory of HPLC/PDA
This module covers the theory of UV/Visible light absorption and the optics of Waters 996, 2996 and 2998 Photo Diode Array (PDA) detectors for HPLC.  The theory section covers Beer's law, how lamp output, solvent background adsorption, and a compound's spectrum affect chromatography sensitivity and linearity.  The optics section explains the major optical components of the HPLC PDA detectors and how they work.  This module is self paced and contains knowledge self assessment sections.

750000682 Operation of HPLC/PDA
This module covers the operational parameters of Waters 2998 Photo Diode Array (PDA) detector for HPLC.  This module shows how to set the appropriate data acquisition rate and filtering constant for a separation.  There is an explanation and example of using both the Median Baseline Filter (MBF) and the Reference Wavelength Compensation function to correct for baseline drift or wander.  This module is self paced and contains a knowledge self assessment section.

750000683 Maintenance of HPLC/PDA   
This module covers routine daily and yearly maintenance of Waters 996, 2996, and 2998 Photo Diode Array (PDA) detectors for HPLC.  This module shows how to properly change the lamp and provides recommended cleaning procedures for the flow cell. This module is self paced.

750000685 Theory of UPLC/TUV
This module covers the theory of UV/Visible light absorption and the optics of Waters Acuity Tunable Ultra Violet (TUV) detector for UPLC.  The theory section covers Beer's law, how lamp output, solvent background adsorption, and a compound's spectrum affect chromatography sensitivity and linearity.  The optics section explains the major optical components of the UPLC TUV detector and how they work. This module is self paced and contains knowledge self assessment sections.

750000686 Operation of UPLC/TUV
This module covers the operational parameters of Waters ACQUITY Tunable Ultra Violet (TUV) detector for UPLC.  This module shows how to set the appropriate data acquisition rate and filtering constant for a separation.  There is an explanation and example of using the Median Baseline Filter (MBF) to correct for baseline drifting.  This module is self paced and contains a knowledge self assessment section.

750000687 Maintenance of UPLC/TUV   
This module covers routine daily and yearly maintenance of Waters ACQUITY Tunable UV/Visible (TUV) detector for UPLC.  This module shows how to properly change the lamp and provides recommended cleaning procedures for the flow cell.  This module is self paced.   

750000688 Theory of UPLC/PDA
This module will cover the theory of UV/Visible light absorption and the optics of Waters ACQUITY Photo Diode Array (PDA) detector for UPLC.  The theory section covers Beer's law, how lamp output, solvent background adsorption, and a compound's spectrum affect chromatography sensitivity and linearity.  The optics section explains the major optical components of the UPLC PDA detector and how they work.  This module is self paced and contains knowledge self assessment sections.

750000689 Operation of UPLC/PDA
This module covers the operational parameters of Waters ACQUITY Photo Diode Array (PDA) detector for UPLC.  This module shows how to set the appropriate data acquisition rate and filtering constant for a separation.  There is an explanation and example of using both the Median Baseline Filter (MBF) and the reference compensation function to correct for baseline drift or wander. This module is self paced and contains a knowledge self assessment section.

750000684 Maintenance of UPLC/PDA 
This module covers routine daily and yearly maintenance of Waters ACQUITY Photo Diode Array (PDA) detector for UPLC.  This module shows how to properly change the lamp and provides recommended cleaning procedures for the flow cell.  This module is self paced.