MassLynx Quadrupole Core eLearning Track
This track contains 29 modules listed below and covers the theory and operation of MassLynx controlled quadrupole-based LC/MS systems. This track covers theoretical and practical aspects of LC method development, LC and MS instrument set up/control and quantitative analysis of LC/MS data.
If you would like to register for individual eLearning courses, please see courses below:
750000658 Binary vs. Quaternary Gradient Systems
This module discusses two types of solvent delivery systems; binary versus quaternary gradient systems. It reviews the similarities and differences between the two systems and how to optimize for UPLC gradient chromatography. The topics covered include hardware components, gradient formation, specification differences, system volume and re-equilibration considerations.
750000659 LC/MS Contamination Issues
This course will discuss how to minimize contamination in LC/MS. It defines what contamination is, where it comes from, with examples, how it can affect your chromatography and spectra, and how to minimize it.
750000660 LC/MS Introduction
This module is designed to introduce the basics of mass measurement and establish the foundation for topics presented in later modules. After addressing the question, "What is LC/MS?" the fundamental concepts of mass accuracy and mass resolution are presented.
750000661 Ionization Modes
The three most common modes of ionization in LC/MS are presented in this module: electrospray, atmospheric pressure chemical ionization (APCI), and atmospheric pressure photo ionization (APPI). The basic mechanism of ionization is explained along with certain experimental factors that can affect analyte response. An understanding of these factors can help guide you to choosing optimal experimental conditions.
750000662 Mass Analyzers
The most common mass analyzers present in current LC/MS instruments are presented in this module: single and tandem quadrupole, time of flight and ion trap. The basic operating principles of each mass analyzer is explained along with the data that can be produced.
750000663 LC Method Development
This module is designed to provide a fundamental understanding of LC method development techniques. As a detector, the mass spectrometer presents certain challenges in using mobile phases and additives that are commonly used in LC methods. The factors affecting the use of these solvents and additives will be explained as well as how to select replacement solvents and additives.
750000690 Fundamentals of LCMS
This module will explain the Fundamental Concepts that will build a foundation for your understanding of the more complex, instrument-specific information that you will see in other modules.
750000691 Basic MassLynx Functions
This course is designed to give new users a fundamental understanding of the software architecture, basic functions and the tools present in MassLynx v4.1.
750000692 Intellistart - Quadrupole Based MS Systems
This eLearning module introduces the user to the powerful, automated capabilities of Intellistart for quadrupole LC/MS systems. These include Resolution Setup, m/z Calibration, Sample Tune, and the LC/MS System Check. In addition, the influence of quadrupole resolving power on ion transmission and method robustness are discussed, along with the ability of Intellistart to create MS acquisition methods.
750000696 TargetLynx - Overview
This module covers an overview of TargetLynx, what it is used for and how to access the application manager. The module also discusses how to set up the sample list and acquire data for use with TargetLynx.
750000697 TargetLynx - Method Setup
This module demonstrates how to develop integration parameters for acquired data and then how to use these parameters to create a basic TargetLynx method from the acquired data.
750000698 TargetLynx - Data Processing and Review
This module gives an overview of how to process samples with a TargetLynx processing method and then how to view the results in the data browser. An overview of how to create a report and output the data is also given.
750000699 TargetLynx - Advanced Topics
This module will discuss how to use TargetLynx for compound confirmation and QC monitoring. Topics include the use of ion ratios, PIC scans, sample flagging, QC monitor and TrendPlot.
750000709 MassLynx - Architecture
This module will show the basic structure of MassLynx and how to navigate the main page to locate Instrument controls and useful tools.
750000710 MassLynx - Data Acquisition
This module will show you how to create a simple sample list for data acquisition. Other topics related to data acquisition, such as autosampler configuration and shutdown methods are also shown.
750000711 MassLynx Data Processing and Review - Chromatogram Window
The Chromatogram window is the primary MassLynx tool used to interact with a chromatogram. This module will show you the more common user performed tasks in the Chromatogram window. Topics range from basic display options to chromatogram integration.
750000712 MassLynx Data Processing and Review - Spectrum
The Spectrum window is the primary MassLynx tool used to interact with mass spectra. This module will show you the more common user performed tasks in the Spectrum window. Additionally, some MassLynx spectrum tools are shown.
750000713 MassLynx Data Processing - Strip Functions
The MassLynx Strip functions provide a way of removing noise and unwanted information in a .raw data file. This module explains the four different methods that are contained in the strip function.
750000773 Practical Tips for UV and PDA Detection
- Background information on TUV and PDA detectors
- UV detection
- What compounds can be detected
- Selecting a wavelength
- Linearity
- Choosing the best working range
- Effect of background absorbance
- Sensitivity
- Effects of method parameters on sensitivity
- Causes of some problems
- Differences between TUV and PDA for quantitation
750000787 UPLC Sample Injection Modes
This module will discuss two types of sample injection: Fixed Loop and Flow Through Needle. The topics to be covered include the injection and wash cycles, the advantages of the different injection modes, how to optimize peak area reproducibility and how to minimize carryover.
750000788 Achieving the Best LC Peak Shape
Good peak shape is crucial in liquid chromatography for the best sensitivity, peak resolution, and reproducibility of peak areas and retention times. Causes of non-ideal peak shapes and how to improve peak shape are discussed.
750000844 ACQUITY Tips and Techniques
Tips and techniques for the proper set up, preparation, running and shutting down of an ACQUITY system are discussed, along with the use of the ACQUITY Console for system evaluation. The process and tools for the migration of HPLC methods to UPLC methods is presented.
750000915 Basic MassLynx Familiarization for LCMS Quadrupole Based Systems
Basic MassLynx familiarization for new LCMS tandem quadrupole users. This module covers simple tasks in MassLynx; new project and sample list creation. This also covers basic data manipulation in the chromatogram and spectum windows for LCMS MRM data.
750000916 Basic Tuning and Calibration for LCMS Quadrupole Based Systems
Basic tuning and calibration familiarization for new LCMS tandem quadrupole users. This module covers set up of the Xevo TQ-S mass spectrometer using IntelliStart; includes basic tuning, MRM method and calibration.
750000917 Basic LC Method Familiarization for LCMS Quadrupole Based Systems
Basic LC method familiarization for new LCMS tandem quadrupole users. This module covers basic set up of the ACQUITY system for use with a Xevo TQ-S mass spectrometer; includes the gradient table and sample manager parameters.
750000918 Basic MS Method Familiarization for LCMS Quadrupole Based Systems
Basic MS method familiarization for new LCMS tandem quadrupole users. This module covers basic set up of MRM methods for use with a Xevo TQ-S mass spectrometer.
750000988 HPLC and UPLC Best Practices and Troubleshooting
This module will cover best practices for HPLC and UPLC users to optimize performance and maximize up-time of the system. The module focuses on prevention and troubleshooting of common LC problems.
750001018 Controlling Contamination in LC and LCMS Systems : Part 1
This module covers best practices for controlling contamination in LC and LCMS systems. Topics include common definitions, tips on preventing contamination and solvent considerations.
750001019 Controlling Contamination in LC and LCMS Systems : Part 2
This module covers best practices for controlling contamination in LC and LCMS systems. Topics include troubleshooting, sources of contamination and cleaning procedures to mitigate contamination.