UPLC eLearning Track
This track contains a series of modules that cover the basics of ultra performance liquid chromatography (UPLC). These modules will discuss the theory, operation and maintenance of UPLC using both a TUV and PDA detector.
If you would like to register for individual eLearning courses, please see courses below:
750000685 - Theory of UPLC/TUV
This module covers the theory of UV/Visible light absorption and the optics of Waters Acuity Tunable Ultra Violet (TUV) detector for UPLC. The theory section covers Beer's law, how lamp output, solvent background adsorption, and a compound's spectrum affect chromatography sensitivity and linearity. The optics section explains the major optical components of the UPLC TUV detector and how they work. This module is self paced and contains knowledge self assessment sections.
750000686 - Operation of UPLC/TUV
This module covers the operational parameters of Waters ACQUITY Tunable Ultra Violet (TUV) detector for UPLC. This module shows how to set the appropriate data acquisition rate and filtering constant for a separation. There is an explanation and example of using the Median Baseline Filter (MBF) to correct for baseline drifting. This module is self paced and contains a knowledge self assessment section.
750000687 - Maintenance of UPLC/TUV
This module covers routine daily and yearly maintenance of Waters ACQUITY Tunable UV/Visible (TUV) detector for UPLC. This module shows how to properly change the lamp and provides recommended cleaning procedures for the flow cell. This module is self paced.
750000688 -Theory of UPLC/PDA
This module will cover the theory of UV/Visible light absorption and the optics of Waters ACQUITY Photo Diode Array (PDA) detector for UPLC. The theory section covers Beer's law, how lamp output, solvent background adsorption, and a compound's spectrum affect chromatography sensitivity and linearity. The optics section explains the major optical components of the UPLC PDA detector and how they work. This module is self paced and contains knowledge self assessment sections.
750000689 - Operation of UPLC/PDA
This module covers the operational parameters of Waters ACQUITY Photo Diode Array (PDA) detector for UPLC. This module shows how to set the appropriate data acquisition rate and filtering constant for a separation. There is an explanation and example of using both the Median Baseline Filter (MBF) and the reference compensation function to correct for baseline drift or wander. This module is self paced and contains a knowledge self assessment section.
750000684 - Maintenance of UPLC/PDA
This module covers routine daily and yearly maintenance of Waters ACQUITY Photo Diode Array (PDA) detector for UPLC. This module shows how to properly change the lamp and provides recommended cleaning procedures for the flow cell. This module is self paced.
750000773 - Practical Tips for UV and PDA Detection
This module will discuss practical tips for optimizing Tunable UV (TUV) and Photodiode Array (PDA) detectors for maximum linearity and sensitivity, rugged and reproducible methods.
750000788 - Achieving the Best LC Peak Shape
Good peak shape is crucial in liquid chromatography for the best sensitivity, peak resolution, and reproducibility of peak areas and retention times. Causes of non-ideal peak shapes and how to improve peak shape are discussed.
750000791 - nanoACQUITY Tips and Tricks
This module discusses how to obtain good, reproducible data from the nanoACQUITY including solvents, plumbing, startup and shutdown; and optimizing sample trapping, gradients and sensitivity.
750000787 - UPLC Sample Injection Modes
This module will discuss two types of sample injection: Fixed Loop and Flow Through Needle. The topics to be covered include the injection and wash cycles, the advantages of the different injection modes, how to optimize peak area reproducibility and how to minimize carryover.
750000838 - Fundamentals of UPLC: Theory & Advantages
The Fundamentals of UPLC discusses the theory of how chromatographic advantages of greater speed, sensitivity, and peak resolution are obtained. Information about column chemistry (particle types, ligands, particle and pore sizes) and how it provides a basis for optimizing separations.
750000844 - ACQUITY Tips and Techniques
Tips and techniques for the proper set up, preparation, running and shutting down of an ACQUITY system are discussed, along with the use of the ACQUITY Console for system evaluation. The process and tools for the migration of HPLC methods to UPLC methods is presented.
750000845 - ACQUITY H-Class Tips and Techniques
Tips and techniques for the proper set up, preparation, running and shutting down of an ACQUITY H-Class system are discussed, along with the use of the ACQUITY H-Class Console for system evaluation. The process and tools for the migration of HPLC methods to UPLC methods is presented.
750000862 - Acquity QDa General Theory and Basic Use
Basic mass spectrometry theory and use of the QDa in Empower 3 Software.
750000858 - Introduction to Supercritical Fluid Techniques
750000859 - UPC2Method Development
750000988 - HPLC and UPLC Best Practices and Troubleshooting
This module will cover best practices for HPLC and UPLC users to optimize performance and maximize up-time of the system. The module focuses on prevention and troubleshooting of common LC problems.