General Liquid Chromatography (LC) eLearning Track
This track contains 7 modules that cover general liquid chromatography (LC) topics and concepts. Topics include the following areas: controlling contamination, binary vs. quaternary gradient systems, sample injection modes, peak shape, and practical tips, practices and troubleshooting.
If you would like to register for individual eLearning courses, please see courses below:
750001018 - Controlling Contamination in LC and LCMS Systems : Part 1
This module covers best practices for controlling contamination in LC and LCMS systems. Topics include common definitions, tips on preventing contamination and solvent considerations.
750001019 - Controlling Contamination in LC and LCMS Systems : Part 2
This module covers best practices for controlling contamination in LC and LCMS systems. Topics include troubleshooting, sources of contamination and cleaning procedures to mitigate contamination.
750000658 - Binary vs. Quaternary Gradient Systems
This module discusses two types of solvent delivery systems; binary versus quaternary gradient systems. It reviews the similarities and differences between the two systems and how to optimize for UPLC gradient chromatography. The topics covered include hardware components, gradient formation, specification differences, system volume and re-equilibration considerations.
750000787 - UPLC Sample Injection Modes
This module will discuss two types of sample injection: Fixed Loop and Flow Through Needle. The topics to be covered include the injection and wash cycles, the advantages of the different injection modes, how to optimize peak area reproducibility and how to minimize carryover.
750000788 - Achieving the Best LC Peak Shape
Good peak shape is crucial in liquid chromatography for the best sensitivity, peak resolution, and reproducibility of peak areas and retention times. Causes of non-ideal peak shapes and how to improve peak shape are discussed.
750000773 - Practical Tips for UV and PDA Detection
This module will discuss practical tips for optimizing Tunable UV (TUV) and Photodiode Array (PDA) detectors for maximum linearity and sensitivity, rugged and reproducible methods.
750000988 - HPLC and UPLC Best Practices and Troubleshooting
This module will cover best practices for HPLC and UPLC users to optimize performance and maximize up-time of the system. The module focuses on prevention and troubleshooting of common LC problems.